ifn α2 Search Results


siifnα  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology siifnα
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Siifnα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/IFN-%CE%B12+siRNA/pmc04336497-105-13-28
Average 90 stars, based on 1 article reviews
siifnα - by Bioz Stars, 2026-10
90/100 stars
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anti ifn α2 monoclonal antibody  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology anti ifn α2 monoclonal antibody
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Anti Ifn α2 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/IFN-%CE%B12+Antibody/pm37467825-90-6-13
Average 92 stars, based on 1 article reviews
anti ifn α2 monoclonal antibody - by Bioz Stars, 2026-10
92/100 stars
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ifn-α2 standard  (PBL Biomedical Laboratories)
90
PBL Biomedical Laboratories ifn-α2 standard
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Ifn α2 Standard, supplied by PBL Biomedical Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/ifn+%CE%B12+standard/pmc03754006-123-1-12
Average 90 stars, based on 1 article reviews
ifn-α2 standard - by Bioz Stars, 2026-10
90/100 stars
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fitc-labelled anti-ifn-α2 monoclonal antibody  (Chromaprobe Inc)
90
Chromaprobe Inc fitc-labelled anti-ifn-α2 monoclonal antibody
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Fitc Labelled Anti Ifn α2 Monoclonal Antibody, supplied by Chromaprobe Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/fitc+labeled+anti+ifn+%CE%B12+mab/pmc03050440-69-20-24
Average 90 stars, based on 1 article reviews
fitc-labelled anti-ifn-α2 monoclonal antibody - by Bioz Stars, 2026-10
90/100 stars
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ifn-α2 subtype  (Cosmo Bio USA)
90
Cosmo Bio USA ifn-α2 subtype
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Ifn α2 Subtype, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/ifn+%CE%B12+80+95+peptide/us07189389-113-51-21
Average 90 stars, based on 1 article reviews
ifn-α2 subtype - by Bioz Stars, 2026-10
90/100 stars
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recombinant human ifn-alpha a (alpha 2a) protein  (Bio-Techne corporation)
93
Bio-Techne corporation recombinant human ifn-alpha a (alpha 2a) protein
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Recombinant Human Ifn Alpha A (Alpha 2a) Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/Recombinant+Human+IFN-alpha+A+(alpha+2a)+Protein/custom%4011100-1%4017507495
Average 93 stars, based on 1 article reviews
recombinant human ifn-alpha a (alpha 2a) protein - by Bioz Stars, 2026-10
93/100 stars
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recombinant human ifn-alpha a protein, cf  (Bio-Techne corporation)
94
Bio-Techne corporation recombinant human ifn-alpha a protein, cf
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Recombinant Human Ifn Alpha A Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/Recombinant+Human+IFN-alpha+A+Protein%2C+CF/bio-techne+corporation___11101-1
Average 94 stars, based on 1 article reviews
recombinant human ifn-alpha a protein, cf - by Bioz Stars, 2026-10
94/100 stars
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ifnα2  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC ifnα2
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Ifnα2, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/ifn+%CE%B12+in+serum/pm33078349-118-309-322
Average 90 stars, based on 1 article reviews
ifnα2 - by Bioz Stars, 2026-10
90/100 stars
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human interferon alpha 2 / ifna2 protein  (Sino Biological)
93
Sino Biological human interferon alpha 2 / ifna2 protein
Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with <t>siIFNα,</t> <t>siIRF-7</t> or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.
Human Interferon Alpha 2 / Ifna2 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/Human+Interferon+alpha+2+%2F+IFNA2+Protein/custom%4013833-hnay%4029124516
Average 93 stars, based on 1 article reviews
human interferon alpha 2 / ifna2 protein - by Bioz Stars, 2026-10
93/100 stars
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ifn-α2 crispr/cas9 ko plasmid  (Santa Cruz Biotechnology)
N/A
CRISPR/Cas9 KO Plasmids consists of IFN-α2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)
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legendplex human ifn-α2 capture bead b3, 13x  (BioLegend)
N/A
LEGENDplex Human IFN-α2 Capture Bead B3, 13X Apps: Multiplex; Size: 100 tests (270 µl)
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ifn-α2  (Aladdin Scientific Corporation)
N/A
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Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with siIFNα, siIRF-7 or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.

Journal: Breast Cancer Research : BCR

Article Title: Targeting interferon response genes sensitizes aromatase inhibitor resistant breast cancer cells to estrogen-induced cell death

doi: 10.1186/s13058-014-0506-7

Figure Lengend Snippet: Elevated level of intracellular IFNα drives constitutive overexpression of IFITM1. (A) ELISA analysis of baseline expression of IFNα in cell lysates and supernatant in MCF-7:5C and MCF-7 cells. Cells (1 x 10 6 ) were seeded in a six-well plate in their standard culture media and after 48 hours cells were harvested and the supernatant and pellets were collected. Cell pellets were then lysed by sonication in RIPA buffer containing protease inhibitors. IFNα was measured in the supernatants and cell lysates by ELISA as described in . All the illustrated data were performed in duplicate and are expressed as mean values of three independent experiments ± SD. (B) Measurement of IFNα mRNA was determined by real-time PCR. Fold change was calculated by means of the ΔΔCT method using PUM1 as an internal control. Values are displayed as relative to MCF-7 cells and are means of triplicate measurements ± SD in three independent experiments. (C) Blockade of type 1 interferon receptor, IFNAR1, using neutralizing antibody MAB1155, in MCF-7 and MCF-7:5C cells. Cells were pretreated with 5 μg/mL anti-IFNAR1/2 for 4 hours and then treated with 20 U/mL human recombinant IFNα for 24 hours. Cells were analyzed by Western blot to assess IFITM1, PLSCR1 and β-actin protein level. Results shown are representative of three independent experiments. The protein levels were quantified using the ImageJ software (downloaded from NIH website ) and normalized as the ratio related to β-actin. * P <0.05 or ** P <0.01 versus control. (D) siRNA knockdown of IRF-7 expression in MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7 and after 24 hours knockdown was verified at the mRNA and protein level via RT-PCR and Western blot analyses. (E) Effect of IRF-7 knockdown on IFNα expression in resistant MCF-7:5C cells. Cells were transiently transfected with siRNA targeting IRF-7. After 24 hours, IFNα mRNA and IFNα protein expression were determined by real-time PCR and ELISA, respectively. Data shown for RT-PCR is expressed as fold change over cells transfected with control siRNA. Values are displayed as means ± SD of three independent experiments performed in triplicate. * P <0.05 or ** P <0.01. (F) The effect of IFNα or IRF-7 knockdown on IFITM1, p-STAT2, STAT2, p-STAT1, STAT1, and IRF-7 protein expression in resistant MCF-7:5C cells. Cells were transiently transfected with siIFNα, siIRF-7 or siCon for 48 hours and Western blot analysis was performed on lysates. Results shown are representative of two independent experiments IFITM1, interferon induced transmembrane protein1; IFNAR1, IFN alpha Type 1 receptor; NIH, National Institutes of Health; PLSCR1, phospholipid scramblase 1; SD, standard deviation.

Article Snippet: The siPLSCR1 (cat# sc-44028), siIFITM1 (cat# sc-44549), siSTAT1 (cat# sc-44123), siSTAT2 (cat# sc-29492), siIFNα (cat# sc-63324), siIRF-7 (cat# 38011) and siRNA negative control (cat# sc-37007) were purchased from Santa Cruz Biotechnology, and siBax and siNoxa were purchased from Thermo Fisher Scientific (Pittsburg, PA, USA).

Techniques: Over Expression, Enzyme-linked Immunosorbent Assay, Expressing, Sonication, Real-time Polymerase Chain Reaction, Control, Recombinant, Western Blot, Software, Knockdown, Transfection, Reverse Transcription Polymerase Chain Reaction, Standard Deviation